Skip to main content
Solved

Simulating Imaging a transparent object (cells, etched surface, ...)


Brian.Catanzaro
Fully Spectral
Forum|alt.badge.img+4

When I encounter a transparent object that I need to view with a microscope, I often use dark field.  This is a 'DC stop' that blocks like coming directly back from the object and only allows rays of NA > 0 to pass.  It highlights the edges of a transparent (phase) object.

I want to perform a similar image simulation using OpticStudio.

I cannot find any ready-to-use analyses for phase objects.  Is there a geometric Image Analysis that allows the object to be phase rather than an absorbing object?

Seems that I can do a simple simulation 'by hand' by using the MTF from an obscured optical system or the PSF from the same and convolving that with the object.

Best answer by Mark.Nicholson

I think you'll need the diffraction image analysis or Image Simulation plots (set to use a diffraction PSF) for this. The surface describing the object is a phase-only surface, and the diffraction analyses will take into account for the phase of the wavefront.

View original
Did this topic help you find an answer to your question?

2 replies

Mark.Nicholson
Luminary
Forum|alt.badge.img+3

I think you'll need the diffraction image analysis or Image Simulation plots (set to use a diffraction PSF) for this. The surface describing the object is a phase-only surface, and the diffraction analyses will take into account for the phase of the wavefront.


  • Visible
  • 23 replies
  • February 25, 2022

Thanks dear Mark, if possible kindly recommend any article for the same.

 


Reply


Cookie policy

We use cookies to enhance and personalize your experience. If you accept you agree to our full cookie policy. Learn more about our cookies.

 
Cookie settings