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Simulating Imaging a transparent object (cells, etched surface, ...)

  • July 6, 2021
  • 2 replies
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Brian.Catanzaro
Fully Spectral
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When I encounter a transparent object that I need to view with a microscope, I often use dark field.  This is a 'DC stop' that blocks like coming directly back from the object and only allows rays of NA > 0 to pass.  It highlights the edges of a transparent (phase) object.

I want to perform a similar image simulation using OpticStudio.

I cannot find any ready-to-use analyses for phase objects.  Is there a geometric Image Analysis that allows the object to be phase rather than an absorbing object?

Seems that I can do a simple simulation 'by hand' by using the MTF from an obscured optical system or the PSF from the same and convolving that with the object.

Best answer by Mark.Nicholson

I think you'll need the diffraction image analysis or Image Simulation plots (set to use a diffraction PSF) for this. The surface describing the object is a phase-only surface, and the diffraction analyses will take into account for the phase of the wavefront.

2 replies

Mark.Nicholson
Luminary
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  • Luminary
  • 911 replies
  • Answer
  • July 8, 2021

I think you'll need the diffraction image analysis or Image Simulation plots (set to use a diffraction PSF) for this. The surface describing the object is a phase-only surface, and the diffraction analyses will take into account for the phase of the wavefront.


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  • 23 replies
  • February 25, 2022

Thanks dear Mark, if possible kindly recommend any article for the same.