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Simulating Imaging a transparent object (cells, etched surface, ...)

  • 6 July 2021
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Userlevel 3
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When I encounter a transparent object that I need to view with a microscope, I often use dark field.  This is a 'DC stop' that blocks like coming directly back from the object and only allows rays of NA > 0 to pass.  It highlights the edges of a transparent (phase) object.


I want to perform a similar image simulation using OpticStudio.


I cannot find any ready-to-use analyses for phase objects.  Is there a geometric Image Analysis that allows the object to be phase rather than an absorbing object?


Seems that I can do a simple simulation 'by hand' by using the MTF from an obscured optical system or the PSF from the same and convolving that with the object.

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Best answer by Mark.Nicholson 8 July 2021, 20:13

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Userlevel 7
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I think you'll need the diffraction image analysis or Image Simulation plots (set to use a diffraction PSF) for this. The surface describing the object is a phase-only surface, and the diffraction analyses will take into account for the phase of the wavefront.

Userlevel 1

Thanks dear Mark, if possible kindly recommend any article for the same.

 

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