Hi,
When I check the “use Centroid” in the Huygens PSF setting, based on the documentation, “Center coordinate” becomes the coordinates of the “geometric image centroid' and there is an offset (“Centroid offset”) between “Center coordinates” and “Centroid coordinates”. I am wondering why “Center coordinates” and “Centroid coordinates” do not have same values. And how is the “geometric image centroid” defined?
Thanks